Part:BBa_K3183018

CD27L Endolysin Residues 1-179 Targeting C. difficile; Codon-Optimized for L. reuteri

This part contains the N-terminal CD27L_1-179. This endolysin has previously been shown to be specific to C. difficile. This endolysin works by cleaving glycopeptides, ¹ (Fig. 1), breaking the cell's peptidoglycan and compromising the structural integrity of its cell wall. Two X-ray crystal structures were solved for the N². These are joined by a linker, which we've added ourselves (Figure 2).

Figure 2: X-ray Crystal Structure of the CD27L N-terminal domain (PDB codes 3QAY

Figure 1: CD27L Catalytic Reaction.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]