Intermediate

Part:BBa_K318027

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


rRFP + rRBS + attP

This composite part is an inverted RBS+mRFP and a P22 attP recombination site. The mRFP is excited at 584nm and emits at 607nm. Using the P22 Int, it is possible to insert the plasmid containing this part into the E. coli K12 genome because K12 contains the P22 attB recombination site. It is composed of the following biobricks: BBa_K199021 and BBa_I11033

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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