Generator

Part:BBa_K318026

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


pCons + attB + RBS + GFP + T

This composite part is a GFP generator plus a constitutive promoter with a P22 attB recombination site. Using the P22 Int and a vector with the attP recombination site, it is possible to insert DNA upstream of the GFP generator. DNA insertion or recombination via P22 Int should end constitutive expression of the GFP. It is composed of the following biobricks: BBa_J23100, BBa_I11032, and BBa_E0240.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 741


[edit]
Categories
Parameters
None