Intermediate

Part:BBa_K318025

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


attB + RBS + GFP + T

This composite part is a GFP generator minus a promoter with a P22 attB recombination site. Using the P22 Int and a vector with the attP recombination site, it is possible to insert DNA upstream of the GFP generator. It is composed of the following biobricks: BBa_I11032 and BBa_E0240.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 698


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Categories
Parameters
None