Generator

Part:BBa_K318024

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


pLac + RBS + Int (P22) + T

This composite part is an inducible P22 Int DNA integrase generator which acts on the attB and attP recombination sites. It is composed of the following biobricks: BBa_K200021, BBa_I11030, and BBa_B1006. A terminator was placed downstream of the Int encoding sequence to end transcription after the integrase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None