Generator

Part:BBa_K318022

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


pLac + RBS + Hin + T

This composite part is an inducible Hin DNA recombinase generator which acts on the hixC recombination sites. It is composed of the following biobricks: BBa_K200021, BBa_J31000, BBa_B1006. A terminator was placed downstream of the Hin encoding sequence to end transcription after the recombinase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None