Generator

Part:BBa_K318010

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


T + araC + pBad + RBS + Cre + T

This composite part is an inducible Cre DNA recombinase generator which acts on the lox recombination sites. It is composed of the following biobricks: BBa_B1006, BBa_I718008, BBa_B1006.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1191
    Illegal BamHI site found at 1642
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
    Illegal AgeI site found at 1328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1008


[edit]
Categories
Parameters
None