Composite

Part:BBa_K317042

Designed by: Yuta Sakai   Group: iGEM10_Tokyo-NoKoGen   (2010-10-27)

Constitutive promoter-RBS-lacI-Double terminator-lacI regulated promoter

This is a promoter unit which is regulated by lacI. Because of the constitutive expression of lacI, this promoter is repressed. This promoter could be induced by adding IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None