Composite

Part:BBa_K317038

Designed by: Yasuhiko SASAKI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-21)

RBS-LuxR-Double term-Plux-Lysis

This device senses the AHL concentration in the media and regulates the expression of downstream genes. The complex of the LuxR protein and the AHL activates the promoter, increasing the rate of transcription. Also, This devicce includes BBa_K112808 at the downstream gene, the auto lysis device which causing lysis of E.coli


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2357
    Illegal NheI site found at 2380
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1962
    Illegal AgeI site found at 2032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 942
    Illegal SapI site found at 2613


[edit]
Categories
Parameters
None