Device

Part:BBa_K317028

Designed by: Yoshiyuki MURAKAMI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-20)

Phototaxis Device (Atractant)

This devicce includes BBa_K317003 which works for phototaxis when it expressed in E.coli. BBa_K317003：This fusion chimera mediate retinal-dependent phototaxis responses by Escherichia coli, establishing that the nine-helix membrane portion of the receptor-transducer complex is a modular functional unit able to signal in heterologous membranes.

This device worked as we expected. Please see the experience section.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1850
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 392
    Illegal AgeI site found at 1652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal BsaI.rc site found at 1367
    Illegal SapI site found at 862
    Illegal SapI.rc site found at 1868


[edit]
Categories
Parameters
n/aPhototaxis Device (Atractant)