Device

Part:BBa_K317027

Designed by: Yuta Sakai   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)

OmpR positive promoter(BBa_R0082)-RBS-Antigen 43-Double terminator

Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.

In this device, the expression of Antigen 43 regulate by OmpR positive promoter. This promoter can be activated by EnvZ.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 2067
    Illegal NgoMIV site found at 2577
    Illegal NgoMIV site found at 2598
    Illegal AgeI site found at 1593
    Illegal AgeI site found at 2337
    Illegal AgeI site found at 2751
    Illegal AgeI site found at 2993
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None