Part:BBa_K317026

Constitutive promoter(BBa_J23109)-RBS-Antigen 43-Double terminator

Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.

In this device, Antigen 43 is expressed constitutively under a weak promoter. The result of measuring OD660 approximately 1 cm from the top of the culture solution is shown below (Fig).

It shows the E. coli, which harbors the plasmid encodes this device, the OD660 decreased. Meanwhile, the control didn’t show remarkable decrease. This means E. coli aggregates from the expression of Antigen 43.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 770
  Illegal NgoMIV site found at 1994
  Illegal NgoMIV site found at 2504
  Illegal NgoMIV site found at 2525
  Illegal AgeI site found at 1520
  Illegal AgeI site found at 2264
  Illegal AgeI site found at 2678
  Illegal AgeI site found at 2920
  
• 1000
  COMPATIBLE WITH RFC[1000]