Coding

Part:BBa_K317007

Designed by: Yuta Sakai   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)

Antigen 43(Wildtype)

Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. This will be the wild type of Antigen 43. This parts contain 6 PstI sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 883
    Illegal PstI site found at 1441
    Illegal PstI site found at 2041
    Illegal PstI site found at 2665
    Illegal PstI site found at 2936
    Illegal PstI site found at 2989
    Illegal NgoMIV site found at 709
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2443
    Illegal NgoMIV site found at 2464
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2617
    Illegal AgeI site found at 2859
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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