Part:BBa_K3165015

araBp

This part is used as an inducible promoter sensitive to varying concentrations of L-Arabinose. araBAD promoter is repressed by the presence of glucose and can be used to regulate gene expression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 120
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Biology

The araBAD promoter of the L-Arabinose operon in Escherichia coli allows tightly controlled, titrable expression of the gene of interest placed downstream the promoter. The promoter is sensitive to small changes in the concentration of arabinose while glucose serves as a repressor.
araC protein acts as the repressor protein which has an affinity to the promoter region in araBAD and suppresses the transcription of the downstream gene. The araBAD promotes has Pc subunit, O1 subunit, CAP binding subunit, I1 + I2 subunit and pBAD promoter region.

Usage

We are using this promoter for the production of CcdB L83S mutant protein (BBa_K3165014) by induction with arabinose. The Generator of CcdB L83S mutant protein (BBa_K3165048) which has a core mutation at the 83^th amino acid residue position uses this promoter as a regulator.

References

1. Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.
   
2. Newman, J. R. & Fuqua, C. (1999). Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.