Composite

Part:BBa_K316024

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-23)

Chloraphenicol resistance gene with dif and 5amyE


This part is identical to BBa_K143023 except for the dif siteBBa_K3160 integrated behind the 5' amye BBa_K143001 integration sequence.

Chloramphenicol acetyltransferase (CAT) BBa_J31005 confers resistance to chloramphenicol, a common lab selection antibiotic. The 5' amyE BBa_K143001 integration sequence allows integration into B. subtilis genome, which disrupts ability to breakdown starch. In order for integration to occur, 3' amyE integration sequence BBa_K143002 is also required.

This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce BBa_K143002.


VectorAMYE5d.PNG



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None