Coding

Part:BBa_K316017

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-25)

LacI operator controlled TEV protease

TEV protease S219P autocatalysis resistant variant BBa_K316012. This part had been reversed for the 3' strand in order to reduce any read-through that may be caused by upstream elements. Expression of TEV protease is under control of enhanced hyperspank promoter BBa_K316000.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 869
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 32
  • 1000
    COMPATIBLE WITH RFC[1000]


Please note all the sequences of this part are reversed to code on 3' strand


[edit]
Categories
Parameters
None