Generator

Part:BBa_K3153000

Designed by: Lijuan Zhai   Group: iGEM19_Worldshaper-Wuhan   (2019-10-02)


GFP generator with arsenic promoter

GFP is expressed under the control of arsenic-responsive promoter. The part comprises of an Arsenic responsive promoter and autorepressor unit (Bba_J33201) upstream of a strong ribosome binding site (Bba_B0030) and a reporter protein GFP(Bba_E0040).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1191


[edit]
Categories
Parameters
None