Part:BBa_K3151030
yhjH + Riboswitch (no terminator loop)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1362
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1344
Overview
The activity of the phosphodiesterase determines the concentration of the cyclic-di-GMP that is bound to the terminator stem loop of the riboswitch. This altogether then determines the production of the green fluorescent protein. We hypothesized that the intracellular concentration of c-di-GMP does not affect the activity of the riboswitch with no terminator (RON), but affects the riboswitch with terminator (ROFF). As seen in (Fig. 1) when YHJH (Phosphodiesterase) was induced and was activated, its activity had no effect on the Riboswitch with no terminator loop (RON) regardless of the cyclic-di-GMP levels, which confirms our hypothesis.
Characterization
Figure 1. Agarose gel of sequence confirmed parts, showing the size (bp) of R ON with YHJH.
Figure 2.The constitutive riboswitch was expressed in DH5ɑ cells. The black lines represent RON samples, the blue lines represent ROFF samples. All RON samples were observed to express more Green Fluorescent Protein (GFP) than corresponding ROFF samples. The effect of sucrose is shown as inhibiting the expression of GFP, shown in the decrease of lower.
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