Reporter

Part:BBa_K315048

Designed by: Anvi Raina   Group: iGEM10_Davidson-MissouriW   (2010-09-22)


pLac+RBS+RFP+RBS+TetA

This construct was built after preliminary experiments suggested that there might be a transcription terminator in the 3' end of the TetA gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1274
    Illegal NgoMIV site found at 1642
    Illegal NgoMIV site found at 1802
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLac+RBS+RFP+RBS+TetA