Measurement

Part:BBa_K315044

Designed by: Curtiss Lane   Group: iGEM10_Davidson-MissouriW   (2010-07-22)


pLacI-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)

This sequence contains the wild type TetA gene which has been deoptimized from base pairs 1 to 290. This composite part has the construct of pLacI-RBS-RFP-RBS-TetA (deoptimized from bp 1-290) and therefore has the ability to produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1130
    Illegal NgoMIV site found at 1498
    Illegal NgoMIV site found at 1658
    Illegal AgeI site found at 637
    Illegal AgeI site found at 749
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLacI-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)