Measurement

Part:BBa_K315042

Designed by: Curtiss Lane   Group: iGEM10_Davidson-MissouriW   (2010-07-22)

pLac-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)

This sequence contains the wild type TetA gene which has been deoptimized from base pairs 1 to 290. This composite part has the construct of pLac-RBS-RFP-RBS-TetA (deoptimized from bp 1-290) and therefore has the ability to produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1274
    Illegal NgoMIV site found at 1642
    Illegal NgoMIV site found at 1802
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLac-RBS-RFP-RBS-TetA (Deoptimized from bp 1-290)