Coding

Part:BBa_K315040

Designed by: Curtiss Lane   Group: iGEM10_Davidson-MissouriW   (2010-07-22)


TetA (Optimized from bp 1to 290)

This sequence is the wild type TetA gene which has been optimized from base pairs 1 to 290. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein. Being that this gene is optimized, we are hypothesizing that it will allow for greater translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 315
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 843
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTetA Fully Optimized (bp 1-290)