Coding

Part:BBa_K315031

Designed by: Danielle Wagner   Group: iGEM10_Davidson-MissouriW   (2010-07-21)


pLac-RBS-RFP-RBS-TetA(Optimized from bp 1-290)

This sequence contains the wild type TetA gene which has been optimized from base pairs 1 to 290. This composite part has the construct of pLac-RBS-RFP-RBS-TetA(Optimized from bp 1-290) and therefore has the ability to produce the Tetracycline resistance protein. Being that this gene is optimized, we are hypothesizing that it will allow for greater translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis). This TetA gene has been optimized from base pairs 1 to 290 using the online Optimizer program (http://genomes.urv.es/OPTIMIZER/) and was synthesized using customized Oligo segments that were annealed and ligated into a pSB1A2 vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1102
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1247
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1273
    Illegal NgoMIV site found at 1641
    Illegal NgoMIV site found at 1801
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLac-RBS-RFP-RBS-TetA(Optimized from bp 1-290)