DNA

Part:BBa_K315030

Designed by: Nitya Rao   Group: iGEM10_Davidson-MissouriW   (2010-07-15)


pTet + loxP forward + RBS + RFP + lox 5171 forward

This construct consists of a promoter followed by a "floxed" RFP gene. Because the two lox sites are not identical, we do not expect RFP to be excised when cre recombinase is introduced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
    Illegal AgeI site found at 789
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None