DNA

Part:BBa_K315025

Designed by: Brianna Pearson   Group: iGEM10_Davidson-MissouriW   (2010-07-15)


pTet-loxBri(F)-RBS-RFP-loxN

This device shows the activity of Cre on two variant lox sites, one with three point mutations (loxBri) in the inverted arm region and one with two (loxN). If these arms are compatible, which they shouldn't be, then RFP will be excised and cells will no longer glow red in the presence of Cre.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None