DNA

Part:BBa_K315023

Designed by: Brianna Pearson   Group: iGEM10_Davidson-MissouriW   (2010-07-15)


pTet-loxBri(F)-RBS-RFP-loxBri(F)

This device shows the activity of Cre on variant lox sites with three point mutations in the inverted arm region. If these arms are compatible, as they should be, then RFP will be excised and cells will no longer glow red in the presence of Cre.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None