Coding

Part:BBa_K315021

Designed by: Danielle Wagner   Group: iGEM10_Davidson-MissouriW   (2010-07-14)


TetA (DeOptimized from bp 144 to 290)

This sequence is the wild type TetA gene which has been deoptimized from base pairs 144 to 290. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein. Being that this gene is deoptimized, we are hypothesizing that it will allow for less translational efficiency as compared to the wild type TetA (no data is available at this time to support or reject this hypothesis)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 684
    Illegal NgoMIV site found at 844
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTetA (DeOptimized from bp 144 to 290)