Coding

Part:BBa_K315020

Designed by: Danielle Wagner   Group: iGEM10_Davidson-MissouriW   (2010-07-14)

TetA (Optimized from bp 144 to 290)

This sequence is the wild type TetA gene which has been optimized from base pairs 144 to 290. This is a basic part that can be placed behind a promoter and produce the Tetracycline resistance protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 315
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 843
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTetA (Optimized from bp 144 to 290)