Reporter

Part:BBa_K315009

Designed by: Jes Testa   Group: iGEM10_Davidson-MissouriW   (2010-06-22)


pLacI-RBS-RFP-RBS-TetA

Short version of pLacI promoter (56 bp) driving expression of monomeric RFP and TetA resistance proteins with strong RBS for each.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1130
    Illegal NgoMIV site found at 1498
    Illegal NgoMIV site found at 1658
    Illegal AgeI site found at 637
    Illegal AgeI site found at 749
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None