Part:BBa_K3150001

Azr

A gene (492bp) coding for a non-specific enzyme from Rhodobacter sphaeroides stain AS1.1737 that is used to degrade a double nitrogen (N=N) azo bond, as found in azo dyes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

SDS-PAGE experiments was performed to verify the expression of the enzyme protein. AzR- represent no induction with IPTG, and AzR+ represent after induction by IPTG. By reviewing the literature, we found that AzR has a protein size of 19.3 kDa. After comparison with the images, we found that Azr were successfully expressed under the induction of IPTG. However, in the previous decolorization experiments, the decolorization ability of AzR+ was not good. We speculated that this may be related to the experimental environment. Decolorization of dyes by AzR+ requires a strict anaerobic environment. We sealed the EP tube with a sealing strip and could not meet the anaerobic requirements of the enzyme.