Part:BBa_K3143681

Tobacco Etch Virus (TEV) Protease (S219V)

TEV is a highly sequence-specific cysteine protease that cleaves the amino-acid sequence ENLYFQG/S between Q and the last amino acid (G/S) (41). The natural TEV protease readily cleaves itself to generate a truncated enzyme with greatly diminished activity^[1], while the TEVS219V mutant is not only far more stable than the natural TEV protease, but also a more efficient catalyst^[2].

References

1. Parks, T.D., Leuther, K.K., Howard, E.D., Johnston, S.A. and Dougherty, W.G. (1994) Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase. Anal. Biochem., 216, 413-417.

2. Kapust, R.B., Tözsér, J., Fox, J.D., Anderson, D.E., Cherry, S., Copeland, T.D. and Waugh, D.S. (2001) Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng., 14, 993-1000.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 319
  Illegal SapI.rc site found at 667