Part ZF21-16-VP64 is a change of Part ZF_21-16KRAB (BBa_K2446039). Both parts are mammalian synthetic transcription factors (SynTFs) based on Cys2-His2 zinc finger 21-16 (ZF_21-16) as protein chassis. However, our part is a change on BBa_K747096 by the replacement of KRAB transcription regulating domain with VP64 transcription regulating domain. So, our part is made up of three core domains from N-terminal to C-terminal: ZF_21-16 DNA binding domain(p58), nuclear location sequence and VP64 transcription regulating domain. The placement of VP64 allows for the activation of expression of our mammalian synthetic promoter CMV-8_ZF_21-16, while KRAB was used to repress the expression of CMV-8_ZF_21-16. At the same time, we changed corresponding mammalian synthetic promoter. When inhibited, the binding sites that KRAB transcription regulating domain specifically binds should be behind the promoter, so the mammalian synthetic promoter CMV-8_ZF_21-16 was before the binding sites. When activated, the binding sites that VP64 transcription regulating domain specifically binds should be before the promoter, so our mammalian synthetic promoter CMV-8_ZF_21-16 was behind the binding sites.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1
- 21Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC