Coding

Part:BBa_K3132000

Designed by: Qiliang Yang   Group: iGEM19_SMMU-China   (2019-10-03)


Gal4-VP64

This part is transformed from the part: Gal4-KRAB(BBa_K2446037) of Igem17_Fudan. To turn this TF into an activating one, we replaced the KRAB domain with a VP64. And this approach successfully reversed its effect as we had expected. Gal4-VP64 containing three core domains from N-terminal to C-terminal: GAL4 DNA binding domain, nuclear location sequence and VP64 transcription regulating domain. And a (G4S) linker was added between DBD and NLS for providing region flexibility. GAL4DBD enable binding to specific DNA sequences, so that we can use Gal4-VP64 as a specific transcription factors to activate the expression of our downstream synthetic promoter elements and minimal CMV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 41
    Illegal NgoMIV site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None