Coding

Part:BBa_K3121007

Designed by: Anish Ganju   Group: iGEM19_IISER_Bhopal   (2019-10-21)


Trigger Factor Frigidicola

The TF was isolated from P. frigidicola, which exists as a monomer in solution (similar to the P. Haloplanktis TF), in direct contrast with the dimeric E. Coli TF. It has also been reported that this TF shows a higher efficiency than the E.Coli TF as regards expression of recombinant protein in cell-free systems - further underscoring its superior appeal.

T--IISERB_Bhopal--Frigidicolalowppx.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 10
    Illegal XbaI site found at 1372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10
    Illegal BglII site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 10
    Illegal XbaI site found at 1372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 10
    Illegal XbaI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1068


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Categories
Parameters
None