Composite
Part:BBa_K3120019
Designed by: Shuiqin Gong Group: iGEM19_BM-AMU (2019-08-14)
5LTR+TRE+pCAG+Snail1+IRES+NeoR+3LTR+AmpR
The main function of this part is to act as an induction expression system. In the presence of DOX, tetR-Krab binds with TRE, thus promoting the transcription of downstream snail1 and promoting the EMT process.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 20
Illegal BglII site found at 3300 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3106
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2
Illegal BsaI site found at 3282
Illegal BsaI site found at 4183
Illegal SapI.rc site found at 2955
Illegal SapI.rc site found at 3165
[edit]
Categories
Parameters
None |