Composite

Part:BBa_K3120017

Designed by: Shuiqin Gong   Group: iGEM19_BM-AMU   (2019-08-14)


N-cad LA+Glycine linker+eGFP+loxP+NeoR+loxP+N-cad RA

This part is the homologous recombination plasmid of N-Cadherin. Using crisper CAS9 technology, homologous recombination can occur between the left arm and right arm of N-Cadherin and the corresponding fragments on the genome, so as to realize the insertion of eGFP and the subsequent Loxp + neomycin resistance gene before the termination codon of N-Cadherin gene of homo sapiens H9(human embryonic stem cell line).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2825
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3509
    Illegal SapI.rc site found at 2674
    Illegal SapI.rc site found at 2884


[edit]
Categories
Parameters
None