Intermediate

Part:BBa_K311003

Designed by: Matthew Adams, Swati Choudhary, Anthony Goering, Rachel Farr, Ethan Johnson, Annie Kathuria, Poonam Srivastava, Ian Windsor   Group: iGEM10_Minnesota   (2010-10-15)


Bacterial Microcompartment Proteins

Three proteins subunits of a bacterial micro-compartment. The genes are in the pSB1C3 plasmid. Three total genes are present, two are together in a single operon and the single gene is upstream from the pair. They are flanked directly upstream by a mutated Lac promoter to make them constitutive. The genes were amplified by PCR from genomic DNA using primers that would introduce restriction sites flanking the genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
    Illegal BglII site found at 689
    Illegal XhoI site found at 484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 901


[edit]
Categories
Parameters
None