Composite

Part:BBa_K311000

Designed by: Matthew Adams, Poonam Srivastava, Ian Windsor   Group: iGEM10_Minnesota   (2010-10-15)

Constitutive tet promoter with mRFP

It is a tet constitutive promoter isolated from a small combinatorial library. The promoter is one of the weakest promoters in the library. The mRFP is placed downstream to the promoter. The activity of the promoter was checked in TOP10 cells, which are negative for tet repressor, and also in DH5 alpha pro cells which have tet repressor gene integrated into the genome. The red fluorescence was measured by FACs with different inducer concentrations in both the cell types. It was observed that the under all the inducer concentrations the promoter activity remains the same.

Fluorescence intensities of RFP with weak promoter
Fluorescence intensities of RFP with strong promoter
Comparison of strong and weak promoters

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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