Composite

Part:BBa_K3103011

Designed by: Nicholas Menhart, Thao Dang, Sarahi Trujillo   Group: iGEM19_IIT_Chicago   (2019-10-16)


SuperPETase gene

The purpose of the superPETase gene is to degrade PET plastic in the ocean through cyanobacteria. It is composed of a TorA sequence, superPETase gene, a mutation on the gene and a myc tag. The main purpose of the TorA sequence export the protein it is linked to past the plasma membrane, to the periplasmic space. This is a twin-arginine signal sequence that has been shown to function in cyanobacteria. Both the PETase gene and TorA gene were obtained from a research paper.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 223
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 241
    Illegal NgoMIV site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
efficiency
protein