Reporter

Part:BBa_K3101000

Designed by: Bryan Bourrat   Group: iGEM19_UniGE-Geneva   (2019-10-16)


CTCF-ERE-SYFP2

The estrogen receptor (ER) is a ligand-activated enhancer protein. ER binds to specific DNA sequences called estrogen response elements (EREs) with high affinity and transactivates gene expression in response to estradiol (E2).

To visualize the activity of this receptor by estrogen induction (fluorescence by activation of ER by E2), we used the bright yellow fluorescent protein : SYFP2, which is a GFP based monomeric protein with optimized folding, maturation and a narrow fluorescence emission spectrum with a maximum of 515 nm. This fluorescent probe can be used to study biological functions on fixed cells or tissues but also to make dynamic measurements on cells in culture or living organisms.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 30
    Illegal XbaI site found at 131
    Illegal XbaI site found at 193
    Illegal SpeI site found at 16
    Illegal SpeI site found at 77
    Illegal SpeI site found at 1289
    Illegal PstI site found at 328
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 235
    Illegal SpeI site found at 16
    Illegal SpeI site found at 77
    Illegal SpeI site found at 1289
    Illegal PstI site found at 328
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 235
    Illegal BglII site found at 83
    Illegal BglII site found at 366
    Illegal BglII site found at 1321
    Illegal XhoI site found at 119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 30
    Illegal XbaI site found at 131
    Illegal XbaI site found at 193
    Illegal SpeI site found at 16
    Illegal SpeI site found at 77
    Illegal SpeI site found at 1289
    Illegal PstI site found at 328
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 30
    Illegal XbaI site found at 131
    Illegal XbaI site found at 193
    Illegal SpeI site found at 16
    Illegal SpeI site found at 77
    Illegal SpeI site found at 1289
    Illegal PstI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
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