Composite

Part:BBa_K310006

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-09-24)

ArsR under the control of J23100, includes RBS B0034 and terminator B0015

ArsR is a transcriptional repressor regulating the Ars Operon in E. coli. This biobrick includes ArsR under the control of the promoter J23100 and includes the RBS B0034 and terminator B0015

This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199


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