Composite
cex-hlyA

Part:BBa_K3093007

Designed by: Hu Shijia   Group: iGEM19_ECUST_China   (2019-10-21)


His tag-cex-hlyA

Usage and Biology

Cellulomonas fimi exo-beta-1,4-glucanase and beta-1,4-xylanase gene（cex) with a his tag and a hlyA tag.

In Cellulomonas fimi the cex gene encodes an exoglucanase (Exg) involved in the degradation of cellulose.The codon usage in C. fimi appears to be quite different from that of Escherichia coli. A dramatic (98.5%) bias occurs for G or C in the third position for the 35 codons utilized in the cex gene.

For more info visit https://www.ncbi.nlm.nih.gov/nuccore/L11080.1

And this part is used in the BBa_K3093009

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 36
    Illegal XhoI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 211
    Illegal NgoMIV site found at 584
    Illegal NgoMIV site found at 1086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 631
    Illegal SapI.rc site found at 714


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Categories
Parameters
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