Composite
cenA-hlyA

Part:BBa_K3093006

Designed by: Hu Shijia   Group: iGEM19_ECUST_China   (2019-10-21)


His tag-cenA-hlyA

Usage and Biology

Cellulomonas fimi cenA gene with a his tag and a hlyA tag.

Cellulomonas fimi cenA gene encoding endoglucanase.They expressed carboxymethylcellulase activity in Escherichia coli.

For more info visit https://www.ncbi.nlm.nih.gov/nuccore/M15823.1


And this part is used in the BBa_K3093008


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 88
    Illegal NotI site found at 1232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1145
    Illegal BamHI site found at 36
    Illegal BamHI site found at 281
    Illegal XhoI site found at 45
    Illegal XhoI site found at 643
    Illegal XhoI site found at 892
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 423
    Illegal NgoMIV site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 327


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Categories
Parameters
None