Coding

Part:BBa_K309015

Designed by: Chris Palmer   Group: iGEM10_Queens-Canada   (2010-10-22)

NpHR::eCFP (for use in C. elegans)

This is an in fram fusion protein of NpHR (BBa_K309019) and eCFP (BBa_K309020). It can be used to simultaneously express (and verify expression of) NpHR.

NB. This part has introns, and IS INTENDED FOR USE IN A C. ELEGANS CHASSIS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 292
    Illegal AgeI site found at 18
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 228


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Categories
Parameters
None