RNA

Part:BBa_K3081105

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)


A meaningless sequence with two flanking BsaI sites,facilitating replacement of intended sgRNA

A meaningless sequence. When it is flanked by J23119 promoter and sgRNA scaffold, the two BsaI site allows for replacement of this meaningless sequence into intended sgRNA sequence by Golden Gate assembly. It length (412bp) which is far longer than normal sgRNA (<20bp) can facilitate distinguishing successful Golden Gate product when the colony PCR fragment is loaded onto gel.For more detailed information, see BBa_K3081058

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 406
    Illegal BsaI.rc site found at 2


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Categories
Parameters
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