Translational_Unit

Part:BBa_K3071020

Designed by: Leung Hei Man   Group: iGEM19_Hong_Kong-CUHK   (2019-10-13)


Xcc Clp-pspF fused with Myc-tag with RBS

This composite part contains the Phage shock protein F transcriptional activation domain fused with cAMP receptor-like protein (pspF TAD - Clp) (BBa_K3071009) with the Ribosome binding site (BBa_J61102)and C-terminus Myc-tag with a double stop codon (BBa_K3071017)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 925
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 928
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 925
    Illegal NgoMIV site found at 1003
    Illegal AgeI site found at 108
    Illegal AgeI site found at 136
    Illegal AgeI site found at 274
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None