Composite

Part:BBa_K307002

Designed by: Joo-han Gwak   Group: iGEM10_CBNU-Korea   (2010-10-27)


rctA-ori2vc-rctB

this part is replication origin of Vibrio cholerae O1 biovar El Tor str. N16961 chromosome II with rctA and rctB replication protein coding genes. rctB gene express replicaiton starting protein and rctA gene regulate RctB protein. we used this part to make mini chromosome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 301
    Illegal BglII site found at 2130
    Illegal BglII site found at 2320
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1549
    Illegal SapI.rc site found at 2087


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Categories
Parameters
None