Coding

Part:BBa_K3061010

Designed by: Kaidi Chen   Group: iGEM19_DUT_China_B   (2019-10-16)


SP_PhyB_GSAT-Linker_C-rluc

It is the combination of signal peptide, C terminal of Renilla luciferase and phytochrome B. SP can target the protein to locate at the membrane; C-rluc can combine with N-rluc while PhyB can combine with Pif3

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 553
    Illegal BamHI site found at 635
    Illegal XhoI site found at 586
    Illegal XhoI site found at 605
    Illegal XhoI site found at 2740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 61
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2125
    Illegal BsaI site found at 2531
    Illegal SapI site found at 802


[edit]
Categories
Parameters
None