Composite

Part:BBa_K3052059

Designed by: Xiaoyun Lu   Group: iGEM19_XJTU-CHINA   (2019-10-16)


CI+Pλ+DHJ+ho1-pcyA

This part is the intermediate product of Golden Gate Assembly of our light-controlling circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2739
    Illegal NheI site found at 2762
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
    Illegal NgoMIV site found at 1050
    Illegal NgoMIV site found at 3852
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None