Composite

Part:BBa_K3052057

Designed by: Xiaoyun Lu   Group: iGEM19_XJTU-CHINA   (2019-10-16)


CI+Pλ+LGF+ho1-pcyA

This part is the intermediate product of Golden Gate Assembly of our light-controlling circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2769
    Illegal NheI site found at 2792
    Illegal PstI site found at 1327
    Illegal PstI site found at 1846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1158
    Illegal BglII site found at 2120
    Illegal BglII site found at 2168
    Illegal BglII site found at 4329
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1327
    Illegal PstI site found at 1846
    Illegal NgoMIV site found at 1479
    Illegal NgoMIV site found at 3882
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None